SubtiBank SubtiBank
Version comparison:

2017-07-05 14:32:582025-04-08 05:32:44

description

anti-SigW

anti-[[protein|SigW]]

locus

BSU01740

BSU_01740

geneLength

624

627

function

control of SigW activity

control of [[protein|SigW]] activity

product

anti-SigW

anti-[[protein|SigW]]

outlinks

bsu

BSU01740

BSU_01740

Gene

Coordinates

195,426 → 196,052

195,426 196,052

The protein

Protein family

anti-sigma-W factor family (according to Swiss-Prot)

zinc-associated anti-sigma factor (ZAS) superfamily (with [[protein|YlaD]], according to UniProt)

anti-sigma-W factor family (single member, according to UniProt)

The protein

[SW|Localization]

membrane (according to Swiss-Prot)

cell membrane (according to UniProt)

Biological materials

Mutant

MGNA-B953 (ybbM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1952 NBRP B. subtilis, Japan]

MGNA-B953 (ybbM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1952 NBRP B. subtilis, Japan]

BKE01740 ([[gene|rsiW]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE01740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGGACAGCTCATTTCATCAC, downstream forward: _UP4_TAAAGCGCGTTAGCCGCTTT

BKK01740 ([[gene|rsiW]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK01740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGGACAGCTCATTTCATCAC, downstream forward: _UP4_TAAAGCGCGTTAGCCGCTTT

Labs working on this gene/protein

[SW|Thomas Wiegert], University of Bayreuth, Germany [http://www.genetik.uni-bayreuth.de/LSGenetik1/Thomas%20Wiegert-Dateien/Thomas%20Wiegert.html Homepage]

References

Reviews

20836086, 22381678

20836086, 22381678, 29343670

References

Original Publications

17020587, 14993308, 16816000, 18599827, 12207695, 16899079, 15130127, 12884008, 12076816, 19889088, 14993308, 28319136

17020587, 14993308, 16816000, 18599827, 12207695, 16899079, 15130127, 12884008, 12076816, 19889088, 14993308, 28319136, 28674070

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Domains]

The cytoplasmic domain is able to inactivate SigW and the extracellular and transmembrane domains are crucial for sensing and transducing the signal that triggers SigW activation (PubMed:15130127). The N-terminus binds the zinc ion and is followed by a long helix (about residues 40-80) that fits into a hydrophobic surface groove on SigW, probably blocking its ability to interact with the -10 and -35 promoter elements (PubMed:28319136).

labs

[SW|Thomas Wiegert], University of Bayreuth, Germany [http://www.genetik.uni-bayreuth.de/LSGenetik1/Thomas%20Wiegert-Dateien/Thomas%20Wiegert.html Homepage]